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Article

In vitro evolution of coenzyme-independent variants from the glmS ribozyme structural scaffold.

Methods (San Diego, Calif.) - 15 Aug 2016

Lau Matthew W L, Ferré-D'Amaré Adrian R

Abstract excerpt

Uniquely among known natural ribozymes that cleave RNA sequence-specifically, the glmS ribozyme-riboswitch employs a small molecule, glucosamine-6-phosphate (GlcN6P) as a catalytic cofactor. In vitro selection was employed to search for coenzyme-independent variants of this ribozyme. In addition to shedding light on the catalytic mechanism of the ribozyme, such variants could resemble the evolutionary ancestors...

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Identifiers

PMID: 27130889
DOI: 10.1016/j.ymeth.2016.04.027
PMCID: PMC4981508

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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