Site-specific mutations of conserved C-terminal residues in aminoglycoside 3'-phosphotransferase II: phenotypic and structural analysis of mutant enzymes.

Abstract excerpt

In order to test the biological importance of amino acids in the C-terminal quarter of aminoglycoside 3'-phosphotransferase II enzyme, seven of the highly conserved residues in this region, His-188, Asp-190, Asp-208, Gly-210, Arg-211, Asp-216 and Asp-220, were changed via site-directed mutagenesis. The phenotype of each mutant was compared to wildtype in terms of antibiotic susceptibilities and enzymatic...

Topics

• Amino Acid Sequence
• Aminoglycosides
• Bacteria
• Blotting, Western
• DNA Transposable Elements
• Kanamycin Kinase
• Kinetics
• Microbial Sensitivity Tests
• Molecular Sequence Data
• Mutagenesis, Site-Directed