Article
Tethered Sir3p nucleates silencing at telomeres and internal loci in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 May 1996
Lustig A J, Liu C, Zhang C, Hanish J P
Abstract excerpt
Rap1p binds to sites embedded within the Saccharomyces cerevisiae telomeric TG1-3 tract. Previous studies have led to the hypothesis that Rap1p may recruit Sir3p and Sir3p-associating factors to the telomere. To test this, we tethered Sir3p adjacent to the telomere via LexA binding sites in the r...
Topics
- Bacterial Proteins
- Binding Sites
- Chromosomes, Fungal
- Fungal Proteins
- GTP-Binding Proteins
- Genotype
- Models, Structural
- Mutagenesis
- Plasmids
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Serine Endopeptidases
- Silent Information Regulator Proteins, Saccharomyces cerevisiae
- Suppression, Genetic
- Telomere
- Trans-Activators
- Transcription, Genetic
- rap GTP-Binding Proteins