Article
SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast.
Genes & development - 1 Jan 1997
Strahl-Bolsinger S, Hecht A, Luo K, Grunstein M
Abstract excerpt
Yeast core telomeric heterochromatin can silence adjacent genes and requires RAP1, SIR2, SIR3, and SIR4 and histones H3 and H4 for this telomere position effect. SIR3 overproduction can extend the silenced domain. We examine here the nature of these multiprotein complexes. SIR2 and SIR4 were immu...
Topics
- Blotting, Western
- Cell Extracts
- Cross-Linking Reagents
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Fungal Proteins
- GTP-Binding Proteins
- Gene Expression Regulation, Fungal
- Heterochromatin
- Histone Deacetylases
- Histones
- Models, Genetic
- Mutation
- Precipitin Tests
- Protein Binding
- Saccharomyces cerevisiae
- Silent Information Regulator Proteins, Saccharomyces cerevisiae
- Sirtuin 2