Article
The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.
Molecular & general genetics : MGG - 24 Apr 1996
Stevens D R, Rochaix J D, Purton S
Abstract excerpt
A chimeric gene composed of the coding sequence of the ble gene from Streptoalloteichus hindustanus fused to the 5' and 3' untranslated regions of the Chlamydomonas reinhardtii nuclear gene RBCS2 has been constructed. Introduction of this chimeric gene into the nuclear genome of C. reinhardtii by...
Topics
- Animals
- Bacterial Proteins
- Base Sequence
- Biomarkers
- Blotting, Southern
- Chlamydomonas reinhardtii
- DNA Primers
- Drug Resistance, Microbial
- Genes, Bacterial
- Genes, Plant
- Molecular Sequence Data
- Oligonucleotides, Antisense
- Phenotype
- Phleomycins
- Polymerase Chain Reaction
- Recombinant Fusion Proteins
- Ribulose-Bisphosphate Carboxylase
- Transfection