Article
A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression.
Genetics - 1 Jan 1997
Cerutti H, Johnson A M, Gillham N W, Boynton J E
Abstract excerpt
We have constructed a dominant selectable marker for nuclear transformation of C. reinhardtii, composed of the coding sequence of the eubacterial aadA gene (conferring spectinomycin resistance) fused to the 5' and 3' untranslated regions of the endogenous RbcS2 gene. Spectinomycin-resistant trans...
Topics
- Animals
- Cell Nucleus
- Chlamydomonas reinhardtii
- DNA, Bacterial
- Drug Resistance, Microbial
- Gene Expression
- Genes, Bacterial
- Nucleotidyltransferases
- Phenotype
- Recombinant Fusion Proteins
- Ribulose-Bisphosphate Carboxylase
