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Article

A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression.

Genetics - 1 Jan 1997

Cerutti H, Johnson A M, Gillham N W, Boynton J E

Abstract excerpt

We have constructed a dominant selectable marker for nuclear transformation of C. reinhardtii, composed of the coding sequence of the eubacterial aadA gene (conferring spectinomycin resistance) fused to the 5' and 3' untranslated regions of the endogenous RbcS2 gene. Spectinomycin-resistant transformants isolated by direct selection (1) contain the chimeric gene(s) stably integrated into the nuclear genome, (2)...

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Identifiers

PMID: 9017393
DOI: 10.1093/genetics/145.1.97
PMCID: PMC1207788

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