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Article

Stable nuclear transformation of Chlamydomonas reinhardtii by using a C. reinhardtii gene as the selectable marker.

Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1990

Mayfield S P, Kindle K L

Abstract excerpt

We have developed a stable nuclear transformation system for the unicellular green alga Chlamydomonas reinhardtii. Transformation was accomplished by introducing the cloned C. reinhardtii oxygen-evolving enhancer protein 1 (OEE1) gene into C. reinhardtii cells by bombardment with DNA-coated tungsten particles. The recipient strain was an OEE1-deficient, nonphotosynthetic, acetate-requiring mutant, which recovered...

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Identifiers

PMID: 2179948
DOI: 10.1073/pnas.87.6.2087
PMCID: PMC53631

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