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Article

Stable nuclear transformation of Chlamydomonas reinhardtii by using a C. reinhardtii gene as the selectable marker.

Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1990

Mayfield S P, Kindle K L

Abstract excerpt

We have developed a stable nuclear transformation system for the unicellular green alga Chlamydomonas reinhardtii. Transformation was accomplished by introducing the cloned C. reinhardtii oxygen-evolving enhancer protein 1 (OEE1) gene into C. reinhardtii cells by bombardment with DNA-coated tungsten particles. The recipient strain was an OEE1-deficient, nonphotosynthetic, acetate-requiring mutant, which recovered...

Topics

Algal Proteins
Base Sequence
Chlamydomonas
Cloning, Molecular
Codon
Crosses, Genetic
Escherichia coli
Genes
Genetic Markers
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Photosynthesis

Plant Proteins

Plasmids

Restriction Mapping

Transformation, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 2179948
DOI: 10.1073/pnas.87.6.2087
PMCID: PMC53631

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2179948-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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