Article
Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons of Escherichia coli.
Nucleic acids research - 1 Jan 2008
DeVito Joseph A
Abstract excerpt
This work describes the novel use of tolC as a selectable/counter-selectable marker for the facile modification of DNA in Escherichia coli. Expression of TolC (an outer membrane protein) confers relative resistance to toxic small molecules, while its absence renders the cell tolerant to colicin E1. These features, coupled with the lambdaredgam recombination system, allow for selection of tolC insertions/deletions...
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