Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii.

doi:10.1007/BF00290652

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Article

Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii.

Molecular & general genetics : MGG - 1 Nov 1991

Newman S M, Gillham N W, Harris E H, Johnson A M, Boynton J E

Abstract excerpt

We have developed an efficient procedure for the disruption of Chlamydomonas chloroplast genes. Wild-type C. reinhardtii cells were bombarded with microprojectiles coated with a mixture of two plasmids, one encoding selectable, antibiotic-resistance mutations in the 16S ribosomal RNA gene and the...

Topics

Animals
Blotting, Southern
Blotting, Western
Chlamydomonas reinhardtii
Chloroplasts
DNA, Protozoan
Electrophoresis, Polyacrylamide Gel
Phenotype
Protozoan Proteins
Restriction Mapping
Transformation, Genetic

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