Article
Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii.
Molecular & general genetics : MGG - 1 Nov 1991
Newman S M, Gillham N W, Harris E H, Johnson A M, Boynton J E
Abstract excerpt
We have developed an efficient procedure for the disruption of Chlamydomonas chloroplast genes. Wild-type C. reinhardtii cells were bombarded with microprojectiles coated with a mixture of two plasmids, one encoding selectable, antibiotic-resistance mutations in the 16S ribosomal RNA gene and the other containing either the atpB or rbcL photosynthetic gene inactivated by an insertion of 0.48 kb of yeast DNA in...
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