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Article

Complementation of a Chlamydomonas reinhardtii mutant using a genomic cosmid library.

Plant molecular biology - 1 Feb 1994

Purton S, Rochaix J D

Abstract excerpt

We report the rescue of an arginine-requiring mutant (arg7-8) of Chlamydomonas reinhardtii by complementation using total DNA from a genomic cosmid library. Using the glass-bead transformation method of Kindle [8] four putative transformants able to grow in the absence of exogenous arginine were obtained from 3 x 10(9) treated cells. Southern blot analysis reveals that at least three of the clones have acquired...

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PMID: 8123795
DOI: 10.1007/BF00024121

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ASL

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