Article
Specialized techniques for site-directed mutagenesis in cyanobacteria.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Clerico Eugenia M, Ditty Jayna L, Golden Susan S
Abstract excerpt
Synechococcus elongatus PCC 7942 is an excellent model system for studying the molecular mechanism of the circadian clock in cyanobacteria. The "plastic" genetic characteristics of this organism have facilitated the development of various methods for mutagenesis of its chromosome. These methods are based on homologous recombination between the chromosome and foreign DNA, introduced to the cyanobacteria by either...
Topics
- Alleles
- Chromosomes, Bacterial
- Circadian Rhythm
- Conjugation, Genetic
- Drug Resistance, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genes, Reporter
- Luciferases
- Mutagenesis, Site-Directed
- Plasmids