Article
The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation.
Molecular and cellular biology - 1 Jun 1994
Nelson J A, Savereide P B, Lefebvre P A
Abstract excerpt
We have cloned and sequenced the CRY1 gene, encoding ribosomal protein S14 in Chlamydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. We isolated a mutant strain resistant t...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Nucleus
- Chlamydomonas reinhardtii
- Cloning, Molecular
- DNA Primers
- DNA, Complementary
- Drug Resistance
- Emetine
- Genetic Markers
- Humans
- Molecular Sequence Data
- Mutagenesis
- Polymerase Chain Reaction
- Rats
- Restriction Mapping