Article
Selectable and inheritable gene silencing through RNA interference in the unicellular alga Chlamydomonas reinhardtii.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
van Dijk Karin, Sarkar Nandita
Abstract excerpt
Reverse genetic approaches have become invaluable tools to tap into the wealth of information provided by sequenced genomes. In 2007, sequencing of the Chlamydomonas reinhardtii genome was completed, and with this an increased demand for the development of reverse genetic strategies for gene analysis. In a variety of organisms, including Chlamydomonas, inverted repeat transgenes have been used to produce strains...
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