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Article

Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker.

Gene - 28 Nov 1996

Sizova I A, Lapina T V, Frolova O N, Alexandrova N N, Akopiants K E, Danilenko V N

Abstract excerpt

The aminoglycoside 3'-phosphotransferase type VIII (APHVIII) encoding gene (aphVIII) from Streptomyces rimosus was introduced by glass-bead high-efficiency transformation into the nuclear genome of green unicellular alga Chlamydomonas reinhardtii for induction of transformants resistant to aminoglycoside antibiotics. The aphVIII structural sequence was flanked by S. rimosus regulatory sequences which failed to...

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PMID: 8973302
DOI: 10.1016/s0378-1119(96)00384-8

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