Article
Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker.
Gene - 28 Nov 1996
Sizova I A, Lapina T V, Frolova O N, Alexandrova N N, Akopiants K E, Danilenko V N
Abstract excerpt
The aminoglycoside 3'-phosphotransferase type VIII (APHVIII) encoding gene (aphVIII) from Streptomyces rimosus was introduced by glass-bead high-efficiency transformation into the nuclear genome of green unicellular alga Chlamydomonas reinhardtii for induction of transformants resistant to aminog...
Topics
- Animals
- Cell Nucleus
- Chlamydomonas reinhardtii
- Codon
- Escherichia coli
- Genes, Bacterial
- Genetic Markers
- Immunoblotting
- Kanamycin Kinase
- Phenotype
- Phosphotransferases (Alcohol Group Acceptor)
- Plasmids
- Streptomyces
- Transformation, Genetic