Article
Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae.
Nucleic acids research - 15 Mar 1996
Maddock J R, Roy J, Woolford J L
Abstract excerpt
We have identified six new genes whose products are necessary for the splicing of nuclear pre-mRNA in the yeast Saccharomyces cerevisiae. A collection of 426 temperature-sensitive yeast strains was generated by EMS mutagenesis. These mutants were screened for pre-mRNA splicing defects by an RNA gel blot assay, using the intron- containing CRY1 and ACT1 genes as hybridization probes. We identified 20...
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