Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A quantitative, high-throughput reverse genetic screen reveals novel connections between Pre-mRNA splicing and 5' and 3' end transcript determinants.

PLoS genetics - 1 Jan 2012

Albulescu Laura-Oana, Sabet Nevin, Gudipati Mohanram, Stepankiw Nicholas, Bergman Zane J, Huffaker Tim C, Pleiss Jeffrey A

Abstract excerpt

Here we present the development and implementation of a genome-wide reverse genetic screen in the budding yeast, Saccharomyces cerevisiae, that couples high-throughput strain growth, robotic RNA isolation and cDNA synthesis, and quantitative PCR to allow for a robust determination of the level of nearly any cellular RNA in the background of ~5,500 different mutants. As an initial test of this approach, we sought...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromatin Assembly and Disassembly
Share to Gene Expression Regulation, Fungal

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to High-Throughput Screening Assays

Share to Oligonucleotide Array Sequence Analysis

Share to RNA Precursors

Share to RNA Splicing

Share to Saccharomyces cerevisiae

Share to Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 22479188
DOI: 10.1371/journal.pgen.1002530
PMCID: PMC3315463

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A quantitative, high-throughput reverse genetic screen reveals novel connections between Pre-mRNA splicing and 5' and 3' end transcript determinants.PMID 22479188Read ↗

Interconnections Between RNA-Processing Pathways Revealed by a Sequencing-Based Genetic Screen for Pre-mRNA Splicing Mutants in Fission Yeast.Semantic proximity Read ↗

Transcript specificity in yeast pre-mRNA splicing revealed by mutations in core spliceosomal components.Semantic proximity Read ↗

Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae.Semantic proximity Read ↗

Workflow for Genome-Wide Determination of Pre-mRNA Splicing Efficiency from Yeast RNA-seq Data.Semantic proximity Read ↗

A Yeast-Based High-Throughput Screening Platform for the Discovery of Novel pre-mRNA Splicing ModulatorsSemantic proximity Read ↗

Genome-wide analysis of pre-mRNA splicing: intron features govern the requirement for the second-step factor, Prp17 in Saccharomyces cerevisiae and Schizosaccharomyces pombe.Semantic proximity Read ↗

Rapid screening of yeast mutants with reporters identifies new splicing phenotypes.Semantic proximity Read ↗

Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations.Semantic proximity Read ↗

Analysis of a splice array experiment elucidates roles of chromatin elongation factor Spt4-5 in splicing.Semantic proximity Read ↗

Sequencing of lariat termini in S. cerevisiae reveals 5' splice sites, branch points, and novel splicing events.Semantic proximity Read ↗

A panoramic view of yeast noncoding RNA processing.Semantic proximity Read ↗

Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.