Article
Identification and characterization of yeast mutants that overcome an experimentally introduced block to splicing at the 3' splice site.
RNA (New York, N.Y.) - 1 Aug 1996
Lin J, Rossi J J
Abstract excerpt
An experimentally introduced secondary structure in exon 2 adjacent to the 3' splice site of a yeast ACT-Escherichia coli lacZ fusion gene abolishes splicing in vivo and inhibits beta-galactosidase production. We have devised a genetic screen to isolate both cis and trans-acting mutants that rest...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Cloning, Molecular
- Gene Dosage
- Genes, Fungal
- Molecular Sequence Data
- Nucleic Acid Conformation
- Phenotype
- Protein Structure, Secondary
- RNA Helicases
- RNA Nucleotidyltransferases
- RNA Splicing
- RNA, Catalytic
- RNA, Fungal
- Regulatory Sequences, Nucleic Acid
- Saccharomyces cerevisiae