Article
Mutations in the alpha-amanitin conserved domain of the largest subunit of yeast RNA polymerase III affect pausing, RNA cleavage and transcriptional transitions.
The EMBO journal - 1 Feb 1996
Thuillier V, Brun I, Sentenac A, Werner M
Abstract excerpt
The alpha-amanitin domain or domain f of the largest subunit of RNA polymerases is one of the most conserved of these enzymes. We have found that the C-terminal part of domain f can be swapped between yeast RNA polymerase II and III. An extensive mutagenesis of domain f of C160, the largest subun...
Topics
- Amanitins
- Amino Acid Sequence
- Base Sequence
- Conserved Sequence
- Genes, Fungal
- Mutagenesis, Site-Directed
- Mutation
- Phenotype
- RNA Polymerase III
- RNA, Fungal
- RNA, Transfer
- Saccharomyces cerevisiae