Article
A universally conserved region of the largest subunit participates in the active site of RNA polymerase III.
The EMBO journal - 1 Aug 1995
Dieci G, Hermann-Le Denmat S, Lukhtanov E, Thuriaux P, Werner M, Sentenac A
Abstract excerpt
The largest subunits of the three eukaryotic nuclear RNA polymerase present extensive sequence homology with the beta' subunit of the bacterial enzymes over five major co-linear regions. Region d is the most highly conserved and contains a motif, (Y/F)NADFDGD(E/Q)M(N/A), which is invariant in all multimeric RNA polymerases. An extensive mutagenesis of that region in yeast RNA polymerase III led to a vast majority...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Conserved Sequence
- DNA, Fungal
- Kinetics
- Molecular Sequence Data
- Mutation
- RNA Polymerase III
- RNA, Fungal