Article
Multiplex ARMS-PCR-RFLP method for high-resolution typing of HLA-DRB1.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Oct 1995
Mitsunaga S, Oguchi T, Moriyama S, Tokunaga K, Akaza T, Tadokoro K, Juji T
Abstract excerpt
A reliable method for high-resolution HLA-DRB1 typing using the combination of group-specific amplification and RFLP analysis is described. Group-specific PCR amplification (multiplex ARMS-PCR) was carried out under the same conditions for all groups using seven different primer pairs divided int...
Topics
- Alleles
- DNA Primers
- Genes, MHC Class II
- Genotype
- HLA-DR Antigens
- HLA-DRB1 Chains
- Histocompatibility Testing
- Humans
- Japan
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length