Article
Multiplex ARMS-PCR-RFLP method for high-resolution typing of HLA-DRB1.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Oct 1995
Mitsunaga S, Oguchi T, Moriyama S, Tokunaga K, Akaza T, Tadokoro K, Juji T
Abstract excerpt
A reliable method for high-resolution HLA-DRB1 typing using the combination of group-specific amplification and RFLP analysis is described. Group-specific PCR amplification (multiplex ARMS-PCR) was carried out under the same conditions for all groups using seven different primer pairs divided into four groups: (1) DR1 and DR10; (2) DR2, DR7 and DR9; (3) DR3 DR5, DR6 and DR8, and (4) DR4. The subsequent...
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