Article
Multiplex ARMS-RFLP: a simple and rapid method for HLA-DR4 subtyping.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Jun 1993
Jawaheer D, Ollier W E, Thomson W
Abstract excerpt
We have developed a simple and rapid non-radioactive technique for HLA-DR4 subtyping. A multiplex ARMS-RFLP (Amplification Refractory Mutation System--Restriction Fragment Length Polymorphism) system allows HLA-DR4 subtyping by analysis of the products of two multiplex ARMS reaction mixtures. For...
Topics
- Alleles
- Arthritis, Rheumatoid
- Autoimmune Diseases
- Base Sequence
- Genotype
- HLA-DR4 Antigen
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Substrate Specificity
- Templates, Genetic