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Article

Multiplex ARMS-RFLP: a simple and rapid method for HLA-DR4 subtyping.

European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Jun 1993

Jawaheer D, Ollier W E, Thomson W

Abstract excerpt

We have developed a simple and rapid non-radioactive technique for HLA-DR4 subtyping. A multiplex ARMS-RFLP (Amplification Refractory Mutation System--Restriction Fragment Length Polymorphism) system allows HLA-DR4 subtyping by analysis of the products of two multiplex ARMS reaction mixtures. For some cases, restriction enzyme digests (Hae II and/or SacII) of the products are analysed. The technique relies on the...

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Identifiers

PMID: 8101729
DOI: 10.1111/j.1744-313x.1993.tb00108.x

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