Article
Comprehensive typing of DQB1 alleles by PCR-RFLP.
Tissue antigens - 1 Apr 1994
Sengar D P, Goldstein R
Abstract excerpt
The protocols represented in this report can resolve all 22 DQB1 alleles. The second exon of DQB1 was subjected to PCR using two group-specific primers to obtain DQB1 group 1 (DQ5 and DQ6) and group 2 (DQ2, DQ3, DQ4) specific amplified products, respectively. Three endonucleases, ApaI, BssHII and...
Topics
- Alleles
- Base Sequence
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
