Article
Comprehensive typing of DQB1 alleles by PCR-RFLP.
Tissue antigens - 1 Apr 1994
Sengar D P, Goldstein R
Abstract excerpt
The protocols represented in this report can resolve all 22 DQB1 alleles. The second exon of DQB1 was subjected to PCR using two group-specific primers to obtain DQB1 group 1 (DQ5 and DQ6) and group 2 (DQ2, DQ3, DQ4) specific amplified products, respectively. Three endonucleases, ApaI, BssHII and NciI, can provide typing of DQ5 and DQ6 based on easy-to-read uncleaved, cleaved and a combination of...
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