Article
HLA-DR8 subtyping by polymerase chain reaction (PCR)-DNA conformation polymorphism (DCP) analysis: a simple and practical genotyping method.
Hiroshima journal of medical sciences - 1 Sept 1996
Fukuda Y, Kimura A, Hoshino S, Shintaku S, Sakaguchi T, Asahara T, Sakaki M, Sumimoto R, Tashiro H, Furukawa M, Ohdan H, Yoshida T, Dohi K
Abstract excerpt
Two hundred Japanese panels were serologically typed for human leukocyte antigen (HLA) - DR to assign 65 HLA-DR8 haplotypes, which were then subdivided into two genotypes, i.e., DRB1*0802 and DRB1*0803, by a polymerase chain reaction (PCR)--based, simple, and practical method. The panels possessing DR8 specificity were firstly subjected to PCR with a couple of primers specifically to amplify their DR52 associated...
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