Article
A simple and rapid method for HLA-DRB and -DQB typing by digestion of PCR-amplified DNA with allele specific restriction endonucleases.
Tissue antigens - 1 Jan 1990
Uryu N, Maeda M, Ota M, Tsuji K, Inoko H
Abstract excerpt
The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, which we previously reported as an efficient and convenient typing technique for accurate definition of the HLA-DQA1 and -DPB1 alleles, is now extended and applied to HLA-DRB and -DQB typing. The second exon of the HLA-DRB (B1 and B3 or B4) and DQB (B1 and B2) genes was selectively amplified from genomic DNAs of 70...
Topics
- Alleles
- Base Sequence
- DNA Restriction Enzymes
- HLA-DQ Antigens
- HLA-DR Antigens
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length