Article
HLA-DRB1 genotyping by modified PCR-RFLP method combined with group-specific primers.
Tissue antigens - 1 Apr 1992
Ota M, Seki T, Fukushima H, Tsuji K, Inoko H
Abstract excerpt
We previously introduced HLA-DQA1, -DPB1 and DQB1 genotyping with the modified PCR-RFLP method using some informative restriction enzymes which have either a single cleavage site or alternatively no cleavage site in the amplified DNA region, depending on the HLA alleles, making reading of RFLP band patterns much easier. In this study, 43 HLA-DRB1 alleles, excluding DRB1*1103 and *1104 for which no restriction...
Topics
- Alleles
- Base Sequence
- Blotting, Southern
- Genes, MHC Class II
- Genotype
- HLA-DR Antigens
- HLA-DRB1 Chains
- Histocompatibility Antigens Class II
- Humans
- Molecular Sequence Data
- Oligonucleotide Probes