Article
Complete HLA-A DNA typing using the PCR-RFLP method combined with allele group- and sequence-specific amplification.
Tissue antigens - 1 Nov 1997
Moribe T, Kaneshige T, Inoko H
Abstract excerpt
We have established a practical method of complete high-resolution typing for all HLA-A alleles using the polymerase chain reaction (PCR)-restriction fragment-length polymorphism (RFLP) technique combined with allele group- and sequence-specific amplification. The second and third exons of the HLA-A gene, in which most allelic variations are observed, were separately amplified by PCRs with 3 and 4 group-specific...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
