Article
Avian sarcoma leukemia virus protease linked to the adjacent Gag polyprotein is enzymatically active.
Virology - 20 Dec 1995
Arad G, Bar-Meir R, Almog N, Chorev M, Kotler M
Abstract excerpt
The activity of avian sarcoma leukemia virus (ASLV) protease (PR) prior to its release from the precursor protein was determined by introducing mutations at the cleavage site between PR and the adjacent upstream nucleocapsid (NC) protein. Gag DNA fragments containing these mutations were cloned into expression vectors and introduced into Escherichia coli in which the ASLV proteins were expressed. The dipeptide...
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