Article
Autoprocessing of the human immunodeficiency virus type 1 protease precursor expressed in Escherichia coli from a synthetic gene.
The Journal of general virology - 1 Mar 1992
Valverde V, Lemay P, Masson J M, Gay B, Boulanger P
Abstract excerpt
A gene encoding an N-terminally extended precursor of 107 residues of the human immunodeficiency virus type 1 protease (PR107) was chemically synthesized and cloned into a bacterial expression vector, under the control of the araB promoter. PR107 was expressed alone or fused in phase to the amino or carboxy terminus of the bacterial beta-galactosidase (beta-gal). The yield of protease and beta-gal was found to be...
Topics
- Amino Acid Sequence
- Endopeptidases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Synthetic
- HIV Protease
- HIV-1
- Lac Operon
- Membrane Proteins
- Microscopy, Immunoelectron
- Molecular Sequence Data