Elongation and folding of nascent ricin chains as peptidyl-tRNA on ribosomes: the effect of amino acid deletions on these processes.

Abstract excerpt

Ricin A-chain was used as a test protein to study the effects of deletion of codons on the ribosomal synthesis, release and chaperone-mediated folding of the proteins. Synthesis of wild-type ricin and five mutant proteins was carried out in an Escherichia coli cell-free coupled transcription/translation system from otherwise identical non-linearized plasmids. The deletions involved small numbers of contiguous...

Topics

• Cell-Free System
• Codon
• Electrophoresis, Polyacrylamide Gel
• Molecular Chaperones
• Mutation
• Peptide Chain Elongation, Translational
• Peptide Chain Termination, Translational
• Peptidyl Transferases
• Protein Biosynthesis
• Protein Folding