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Article

Rapid and sensitive method of quantitation of bone gla protein mRNA using competitive polymerase chain reaction.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Mar 1993

Araki N, Robinson F D, Nishimoto S K

Abstract excerpt

A method for sensitive quantitation of bone gla protein (BGP, osteocalcin) mRNA has been developed using competitive polymerase chain reaction after reverse transcription (competitive RT-PCR). The complementary DNA (cDNA) were transcribed from sample RNA was co-amplified in a PCR with a known amount of mutant BGP cDNA (competitor) using the identical oligonucleotide primers. The mutant cDNA with its unique...

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PMID: 8456587
DOI: 10.1002/jbmr.5650080308

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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