Rapid and sensitive method of quantitation of bone gla protein mRNA using competitive polymerase chain reaction.

doi:10.1002/jbmr.5650080308

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Article

Rapid and sensitive method of quantitation of bone gla protein mRNA using competitive polymerase chain reaction.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Mar 1993

Araki N, Robinson F D, Nishimoto S K

Abstract excerpt

A method for sensitive quantitation of bone gla protein (BGP, osteocalcin) mRNA has been developed using competitive polymerase chain reaction after reverse transcription (competitive RT-PCR). The complementary DNA (cDNA) were transcribed from sample RNA was co-amplified in a PCR with a known amo...

Topics

Animals
Base Sequence
Bone and Bones
Cells, Cultured
DNA
Electrophoresis, Agar Gel
Molecular Sequence Data
Mutation
Osteocalcin
Polymerase Chain Reaction
RNA, Messenger
Rats
Rats, Inbred F344
Transcription, Genetic

Source

Open in PubMed

Identifiers

PMID: 8456587
DOI: 10.1002/jbmr.5650080308

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8456587-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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