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Article

Quantitation of matrix Gla protein mRNA by competitive polymerase chain reaction using glyceraldehyde-3-phosphate dehydrogenase as an internal control.

Gene - 3 Apr 1995

Zhao J, Araki N, Nishimoto S K

Abstract excerpt

Matrix Gla (gamma-carboxyglutamic acid) protein (MGP) is a vitamin-K-dependent extracellular matrix protein. A method was developed to quantitate MGP mRNA based on competitive polymerase chain reaction following reverse transcription (competitive RT-PCR). The MGP cDNA was coamplified with a mutant MGP cDNA (competitor). The ratio of MGP to competitor after the PCR reaction was compared to standards to determine...

Topics

Animals
Base Sequence
Calcium-Binding Proteins
Cell Line
DNA, Complementary
Extracellular Matrix Proteins
Glyceraldehyde-3-Phosphate Dehydrogenases
Kidney
Molecular Sequence Data
Mutation
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Messenger

Rats

Matrix Gla Protein

Source

Identifiers

PMID: 7721085
DOI: 10.1016/0378-1119(94)00895-y

Genes

MGP
GAPDH

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7721085-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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