Article
Measurement of gene expression by multiplex competitive polymerase chain reaction.
Analytical biochemistry - 1 Sept 1993
Apostolakos M J, Schuermann W H, Frampton M W, Utell M J, Willey J C
Abstract excerpt
We have developed a polymerase chain reaction (PCR)-based method to measure glutathione peroxidase (GSH-Px) mRNA levels. Expression was measured by multiplex competitive PCR amplification of (a) cDNA from GSH-Px and the "housekeeping" gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and (b) two internal standards consisting of single-base mutants of GSH-Px and GAPDH cDNA that cause either a loss (GSH-Px) or...
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