Article
Measurement of gene expression by multiplex competitive polymerase chain reaction.
Analytical biochemistry - 1 Sept 1993
Apostolakos M J, Schuermann W H, Frampton M W, Utell M J, Willey J C
Abstract excerpt
We have developed a polymerase chain reaction (PCR)-based method to measure glutathione peroxidase (GSH-Px) mRNA levels. Expression was measured by multiplex competitive PCR amplification of (a) cDNA from GSH-Px and the "housekeeping" gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and (b)...
Topics
- Base Sequence
- Bronchi
- Cells, Cultured
- DNA, Complementary
- Densitometry
- Ethidium
- Gene Amplification
- Gene Expression
- Glutathione Peroxidase
- Glyceraldehyde-3-Phosphate Dehydrogenases
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Reproducibility of Results
- Templates, Genetic