Article
Accurate measurement of the relative abundance of different DNA species in complex DNA mixtures.
DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Jun 2012
Jeong Sangkyun, Yu Hyunjoo, Pfeifer Karl
Abstract excerpt
A molecular tool that can compare the abundances of different DNA sequences is necessary for comparing intergenic or interspecific gene expression. We devised and verified such a tool using a quantitative competitive polymerase chain reaction approach. For this approach, we adapted a competitor array, an artificially made plasmid DNA in which all the competitor templates for the target DNAs are arranged with a...
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