Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Accurate and absolute quantitative measurement of gene expression by single-tube RT-PCR and HPLC.

Genome research - 1 Dec 1995

Hayward-Lester A, Oefner P J, Sabatini S, Doris P A

Abstract excerpt

We report a method that allows accurate, absolute quantification of gene expression in a single reverse transcriptase (RT)-PCR reaction. This method makes use of novel high-performance liquid chromatography (HPLC) technology to resolve and quantify the products of competitive, mutant RNA PCRs. The HPLC technique allows rapid, high resolution of reaction products. On-line UV detection eliminates the need for...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Chromatography, High Pressure Liquid
Share to DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Expression

Share to Molecular Sequence Data

Share to Mutation

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 8808471
DOI: 10.1101/gr.5.5.494

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Accurate and absolute quantitative measurement of gene expression by single-tube RT-PCR and HPLC.PMID 8808471Read ↗

Accurate quantitative RT-PCR for relative expression of Slo splice variants.Semantic proximity Read ↗

A high-throughput gene expression analysis technique using competitive PCR and matrix-assisted laser desorption ionization time-of-flight MS.Semantic proximity Read ↗

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.Semantic proximity Read ↗

Single nucleotide primer extension: quantitative range, variability, and multiplex analysis.Semantic proximity Read ↗

Analysis of variation in relative mRNA abundance for specific gene transcripts in single bovine oocytes and early embryos.Semantic proximity Read ↗

Optimization of Capillary-Based Western Blotting for MYO7A.Semantic proximity Read ↗

Isolation and analysis of amplified cDNA fragments during detection of unknown polymorphisms with temperature modulated heteroduplex chromatography.Semantic proximity Read ↗

Measurement of gene expression by multiplex competitive polymerase chain reaction.Semantic proximity Read ↗

Application for PCR technology to subtractive cDNA cloning: identification of genes expressed specifically in murine plasmacytoma cells.Semantic proximity Read ↗

Quantitative RT-PCR-based analysis of allele-specific gene expression.Semantic proximity Read ↗

MOLECULAR CLONING OF AN IL-8-LIKE CXC CHEMOKINE AND TISSUE FACTOR IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) BY USE OF SUPPRESSION SUBTRACTIVE HYBRIDIZATIONSemantic proximity Read ↗

Rapid and sensitive method of quantitation of bone gla protein mRNA using competitive polymerase chain reaction.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.