Hypermutation is observed only in antibody H chain V region transgenes that have recombined with endogenous immunoglobulin H DNA: implications for the location of cis-acting elements required for somatic mutation.

doi:10.1084/jem.177.3.797

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Hypermutation is observed only in antibody H chain V region transgenes that have recombined with endogenous immunoglobulin H DNA: implications for the location of cis-acting elements required for somatic mutation.

The Journal of experimental medicine - 1 Mar 1993

Giusti A M, Manser T

Abstract excerpt

Mice with transgenes containing an antibody H chain V region (VHDJH) gene were used in an analysis of the cis-acting elements required for hypermutation of immunoglobulin (Ig) V genes. These transgenes can somatically recombine with endogenous IgH DNA, leading to the formation of functional heavy...

Topics

Animals
Base Sequence
Chromosome Mapping
DNA
Enhancer Elements, Genetic
Female
Gene Expression Regulation, Neoplastic
Genetic Vectors
Hybridomas
Immunoglobulin Heavy Chains
Immunoglobulin Variable Region

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Male

Mice

Mice, Transgenic

Molecular Sequence Data

Mutation

Promoter Regions, Genetic

Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 8436910
DOI: 10.1084/jem.177.3.797
PMCID: PMC2190935

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8436910-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Hypermutation is observed only in antibody H chain V region transgenes that have recombined with endogenous immunoglobulin H DNA: implications for the location of cis-acting elements required for somatic mutation.PMID 8436910Read ↗

Characterization of the cis-acting elements required for somatic hypermutation of murine antibody V genes using conventional transgenic and transgene homologous recombination approaches.Semantic proximity Read ↗

Somatic generation of hybrid antibody H chain genes in transgenic mice via interchromosomal gene conversion.Semantic proximity Read ↗

Targeted insertion of a variable region gene into the immunoglobulin heavy chain locus.Semantic proximity Read ↗

Analysis of hypermutation in immunoglobulin heavy chain passenger transgenes.Semantic proximity Read ↗

Evaluation of the role of the 3'alpha heavy chain enhancer [3'alpha E(hs1,2)] in Vh gene somatic hypermutation.Semantic proximity Read ↗

Somatic hypermutation of an immunoglobulin mu heavy chain transgene.Semantic proximity Read ↗

Rearrangement of only one human IGHV gene is sufficient to generate a wide repertoire of antigen specific antibody responses in transgenic mice.Semantic proximity Read ↗

A lambda 1 transgene under the control of a heavy chain promoter and enhancer does not undergo somatic hypermutation.Semantic proximity Read ↗

A pivotal role for DNase I-sensitive regions 3b and/or 4 in the induction of somatic hypermutation of IgH genes.Semantic proximity Read ↗

The transcriptional promoter regulates hypermutation of the antibody heavy chain locus.Semantic proximity Read ↗

Somatic hypermutation of an immunoglobulin transgene in kappa transgenic mice.Semantic proximity Read ↗

Mapping the upstream boundary of somatic mutations in rearranged immunoglobulin transgenes and endogenous genes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.