Somatic generation of hybrid antibody H chain genes in transgenic mice via interchromosomal gene conversion.

doi:10.1084/jem.179.1.235

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Article

Somatic generation of hybrid antibody H chain genes in transgenic mice via interchromosomal gene conversion.

The Journal of experimental medicine - 1 Jan 1994

Giusti A M, Manser T

Abstract excerpt

We have constructed lines of mice with transgenes containing an antibody heavy (H) chain variable region (VHDJH) gene and various amounts of natural immunoglobulin (Ig) and plasmid flanking DNA. In these lines, recombination of the transgene and the endogenous Igh locus takes place in B cells, le...

Topics

Alleles
Animals
Blotting, Southern
Cell Line
Chromosomes
Crossing Over, Genetic
DNA
Electrophoresis, Agar Gel
Electrophoresis, Gel, Pulsed-Field
Gene Conversion
Immunoglobulin Heavy Chains
Immunoglobulin Variable Region

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

In Situ Hybridization, Fluorescence

Mice

Mice, Transgenic

Models, Genetic

Polymerase Chain Reaction

Recombination, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 8270869
DOI: 10.1084/jem.179.1.235
PMCID: PMC2191318

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8270869-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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