Article
A lambda 1 transgene under the control of a heavy chain promoter and enhancer does not undergo somatic hypermutation.
European journal of immunology - 1 Jul 1994
Hengstschläger M, Williams M, Maizels N
Abstract excerpt
To identify cis-acting elements responsible for targeting somatic hypermutation to immunoglobulin variable regions, we generated transgenic mice which carry a rearranged lambda 1 gene regulated by the heavy chain intron enhancer, E mu, and the heavy chain promoter PH186.2 from the VH186.2 variabl...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- DNA Mutational Analysis
- Enhancer Elements, Genetic
- Hybridomas
- Immunoglobulin Heavy Chains
- Immunoglobulin lambda-Chains
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Promoter Regions, Genetic