Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation.

doi:10.1093/emboj/17.17.5112

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Article

Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation.

The EMBO journal - 1 Sept 1998

Tiebel B, Aung-Hilbrich L M, Schnappinger D, Hillen W

Abstract excerpt

We constructed and characterized four Tet repressor (TetR) variants with engineered cysteine residues which can form disulfide bonds and are located in regions where conformational changes during induction by tetracycline (tc) might occur. All TetR mutants show nearly wild-type activities in vivo...

Topics

Cysteine
Cystine
Dimerization
Disulfides
Gene Expression Regulation
Motion
Mutation
Operator Regions, Genetic
Protein Binding
Protein Engineering
Protein Structure, Secondary
Repressor Proteins

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