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Article

Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation.

The EMBO journal - 1 Sept 1998

Tiebel B, Aung-Hilbrich L M, Schnappinger D, Hillen W

Abstract excerpt

We constructed and characterized four Tet repressor (TetR) variants with engineered cysteine residues which can form disulfide bonds and are located in regions where conformational changes during induction by tetracycline (tc) might occur. All TetR mutants show nearly wild-type activities in vivo, and the reduced proteins also show wild-type activities in vitro. Complete and reversible disulfide bond formation...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 9724647
DOI: 10.1093/emboj/17.17.5112
PMCID: PMC1170839

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Source receipt: pubmed, retrieved 7 Sept 2026.

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