Article
The role of the N terminus in Tet repressor for tet operator binding determined by a mutational analysis.
The Journal of biological chemistry - 25 Jan 1992
Berens C, Altschmied L, Hillen W
Abstract excerpt
The N-terminal residues preceding the alpha-helix-turn-alpha-helix motif on the Tn10 Tet repressor protein were probed by oligonucleotide-directed deletion mutagenesis for their role in protein activity. All deletion mutants showed decreased repression in vivo, emphasizing the importance of the N...
Topics
- Alleles
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- Chromosome Deletion
- DNA Transposable Elements
- Escherichia coli
- Genes, Bacterial
- Kinetics
- Models, Structural
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Operon
- Protein Conformation
- Recombinant Proteins
- Repressor Proteins
- beta-Galactosidase