Article
New architectures for Tet-on and Tet-off regulation in Staphylococcus aureus.
Applied and environmental microbiology - 1 Feb 2010
Stary Elena, Gaupp Rosmarie, Lechner Sabrina, Leibig Martina, Tichy Evelyn, Kolb Martina, Bertram Ralph
Abstract excerpt
Inducible expression is a valuable approach for the elucidation of gene functions. Here, we present new configurations of the tetracycline-dependent gene regulation (tet) system for Staphylococcus aureus. To provide improved and expanded modes of control, strains and plasmids were constructed for the constitutive expression of tetR or a variant allele, rev-tetR(r2). The encoded regulators respond differently to...
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