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HLA-DQB1 typing of north east Italian IDDM patients using amplified DNA, oligonucleotide probes and a rapid DNA-enzyme immunoassay (DEIA).

Molecular immunology - 1 Jan 1993

Tosi G, Mantero G, Magalini A R, Primi D, Soffiati M, Pinelli L, Sartoris S, Tridente G, Accolla R S

Abstract excerpt

We report on HLA-DQB1 typing in IDDM patients of north east Italian region using an enzymatic method based on the detection of hybridization reaction between PCR amplified DNA from whole blood and allele specific oligonucleotides by an antibody directed against double stranded DNA (DNA-enzyme immunoassay or DEIA). The method is reliable, simple and sensitive as the classical radioactive method with the advantage...

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PMID: 8417376
DOI: 10.1016/0161-5890(93)90427-d

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