Article
Rapid DNA typing of class II HLA antigens using the polymerase chain reaction and reverse dot blot hybridization.
Tissue antigens - 1 Jan 1993
Buyse I, Decorte R, Baens M, Cuppens H, Semana G, Emonds M P, Marynen P, Cassiman J J
Abstract excerpt
A nonisotopic oligotyping method using reverse dot blot hybridization was developed for HLA class II DQA1, DQB1, DPB1, DRB1, DRB3, DRB4, DRB5 alleles. The polymorphic second exon of the different genes was amplified by the polymerase chain reaction (PCR). For each gene the amplified DNA was hybridized at stringent conditions to membrane-bound sequence-specific oligonucleotides (SSOs) and visualization of positive...
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