Article
Analysis of MHC class II antigens in Japanese IDDM by a novel HLA-typing method, hybridization protection assay.
Diabetes research and clinical practice - 1 Mar 1994
Maruyama T, Shimada A, Kasuga A, Kasatani T, Ozawa Y, Ishii M, Takei I, Suzuki Y, Kobayashi A, Takeda S
Abstract excerpt
We examined HLA Class II antigens in 116 Japanese IDDM patients [84 typical IDDM (T-IDD); 32 slowly progressive IDDM (S-IDD)] by the hybridization protection assay (HPA) which is a novel HLA typing method based on hybridization of acridinium-ester-labeled DNA probes to amplified DNA. We detected HLA-DRB1, -DQA1 and -DQB1 genes by this method which is capable of analyzing over 50 samples within 4 h with high...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
