Article
The yeast VPS17 gene encodes a membrane-associated protein required for the sorting of soluble vacuolar hydrolases.
The Journal of biological chemistry - 5 Jan 1993
Köhrer K, Emr S D
Abstract excerpt
vps17 mutants missort and secrete several vacuolar hydrolases. To analyze the role of the VPS17 gene in vacuolar protein delivery, we have cloned this gene by complementation of the vacuolar protein sorting defects of a vps17-5 mutant. Disruption of the VPS17 gene had no effect on the viability o...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Fractionation
- Cloning, Molecular
- DNA, Fungal
- Fungal Proteins
- Genes, Fungal
- Genotype
- Hydrolases
- Membrane Proteins
- Molecular Sequence Data
- Oligodeoxyribonucleotides
- Protein Conformation
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Subcellular Fractions
- Vacuoles