Article
Direct detection of major histocompatibility complex class I binding to antigenic peptides using surface plasmon resonance. Peptide immobilization and characterization of binding specificity.
The Journal of biological chemistry - 25 Jul 1993
Khilko S N, Corr M, Boyd L F, Lees A, Inman J K, Margulies D H
Abstract excerpt
We have developed model systems in which the binding of purified, genetically engineered, soluble analogues of major histocompatibility complex (MHC) class I molecules to immobilized antigenic peptides can be monitored in real time using surface plasmon resonance (SPR). Synthetic analogues of sev...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Binding, Competitive
- Biosensing Techniques
- Cytomegalovirus
- Electromagnetic Phenomena
- H-2 Antigens
- HLA-A Antigens
- Histocompatibility Antigens Class I
- Humans
- L Cells
- Mice
- Molecular Sequence Data
- Peptides
- Protein Binding
- Viral Proteins