Article
Mapping epitopes and antigenicity by site-directed masking.
Proceedings of the National Academy of Sciences of the United States of America - 13 Jun 2006
Paus Didrik, Winter Greg
Abstract excerpt
Here we describe a method for mapping the binding of antibodies to the surface of a folded antigen. We first created a panel of mutant antigens (beta-lactamase) in which single surface-exposed residues were mutated to cysteine. We then chemically tethered the cysteine residues to a solid phase, thereby masking a surface patch centered on each cysteine residue and blocking the binding of antibodies to this region...
Topics
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Antigens, Surface
- Epitope Mapping
- Freund's Adjuvant
- Immunization
- Mice
- Models, Molecular
- Mutation
- Protein Structure, Tertiary
- Rabbits
- beta-Lactamases