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Article

Detection of conformational changes in an immobilized protein using surface plasmon resonance.

Analytical chemistry - 15 May 1998

Sota H, Hasegawa Y, Iwakura M

Abstract excerpt

Utilizing surface plasmon resonance (SPR), we have developed novel methodology for the detection of conformational change(s) in immobilized proteins. A genetically altered E. coli dihydrofolate reductase (DHFR-ASC) was attached to a carboxymethyldextran matrix layer covering the sensor surface of an SPR biosensor through a disulfide linkage at the engineered protein's C-terminus. The DHFR-ASC-immobilized surface...

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Source

Identifiers

PMID: 9608841
DOI: 10.1021/ac9713666

Genes

SPR
DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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