Detection of conformational changes in an immobilized protein using surface plasmon resonance.

doi:10.1021/ac9713666

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Article

Detection of conformational changes in an immobilized protein using surface plasmon resonance.

Analytical chemistry - 15 May 1998

Sota H, Hasegawa Y, Iwakura M

Abstract excerpt

Utilizing surface plasmon resonance (SPR), we have developed novel methodology for the detection of conformational change(s) in immobilized proteins. A genetically altered E. coli dihydrofolate reductase (DHFR-ASC) was attached to a carboxymethyldextran matrix layer covering the sensor surface of...

Topics

Biosensing Techniques
Circular Dichroism
Dextrans
Enzymes, Immobilized
Escherichia coli
Hydrogen-Ion Concentration
Mutation
Protein Conformation
Protein Denaturation
Protein Engineering
Refractometry
Tetrahydrofolate Dehydrogenase

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