Article
Functional domains of the Gs alpha subunit: role of the C-terminus in the receptor-dependent and receptor-independent activation.
Journal of receptor research - 1 Jan 1993
Pantaloni C, Audigier Y
Abstract excerpt
We have developed a rapid and simple model for studying functional domains of Gs alpha subunit, the GTP binding protein involved in adenylyl cyclase activation. Cyc- membranes prepared from a variant S49 cell line which does not express the alpha subunit of Gs are reconstituted by the in vitro tr...
Topics
- Adenosine Diphosphate Ribose
- Adenylyl Cyclases
- Catalysis
- Cholera Toxin
- GTP-Binding Proteins
- Guanosine 5'-O-(3-Thiotriphosphate)
- Humans
- Mutation
- Peptide Fragments
- Protein Biosynthesis
- Receptors, Adrenergic, beta
- Recombinant Proteins