Article
Functional domains of the Gs alpha subunit: role of the C-terminus in the receptor-dependent and receptor-independent activation.
Journal of receptor research - 1 Jan 1993
Pantaloni C, Audigier Y
Abstract excerpt
We have developed a rapid and simple model for studying functional domains of Gs alpha subunit, the GTP binding protein involved in adenylyl cyclase activation. Cyc- membranes prepared from a variant S49 cell line which does not express the alpha subunit of Gs are reconstituted by the in vitro translated Gs alpha subunit. Since the messenger RNA used for in vitro translation is generated from in vitro...
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