Expression and analysis of Gs alpha mutants with decreased ability to activate adenylylcyclase.

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Article

Expression and analysis of Gs alpha mutants with decreased ability to activate adenylylcyclase.

The Journal of biological chemistry - 25 Aug 1991

Itoh H, Gilman A G

Abstract excerpt

We have constructed mutants of the alpha subunit of Gs in an attempt to identify sites in the protein that are important for its interaction with adenylylcyclase. Some residues specific for those G proteins that activate adenylylcyclase were replaced with residues characteristic of Gi alpha. Mutant proteins were expressed in Escherichia coli, and two of these were purified to homogeneity and characterized in...

Topics

Adenylyl Cyclases
Animals
Base Sequence
Blotting, Western
Cattle
Cell Membrane
Cloning, Molecular
DNA
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

GTP-Binding Proteins

Guanosine 5'-O-(3-Thiotriphosphate)

Guanosine Triphosphate

Hydrolysis

Molecular Sequence Data

Mutagenesis, Site-Directed

Mutation

Source

Open in PubMed

Identifiers

PMID: 1651937

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1651937-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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