Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity.

Abstract excerpt

Binding of GTP induces alpha subunits of heterotrimeric G proteins to take on an active conformation, capable of regulating effector molecules. We expressed epitope-tagged versions of the alpha subunit (alpha s) of Gs in genetically alpha s-deficient S49 cyc- cells. Addition of a hemagglutinin (HA) epitope did not alter the ability of wild type alpha s to mediate hormonal stimulation of adenylyl cyclase or to...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Cell Line
• Share to Epitopes