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Article

Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity.

The Journal of cell biology - 1 Dec 1992

Levis M J, Bourne H R

Abstract excerpt

Binding of GTP induces alpha subunits of heterotrimeric G proteins to take on an active conformation, capable of regulating effector molecules. We expressed epitope-tagged versions of the alpha subunit (alpha s) of Gs in genetically alpha s-deficient S49 cyc- cells. Addition of a hemagglutinin (H...

Topics

Adenylyl Cyclases
Base Sequence
Cell Compartmentation
Cell Line
Cholera Toxin
Epitopes
GTP Phosphohydrolases
GTP-Binding Proteins
Isoproterenol
Membrane Proteins
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Precipitin Tests

Propranolol

Signal Transduction

Subcellular Fractions

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Identifiers

PMID: 1280272
DOI: 10.1083/jcb.119.5.1297
PMCID: PMC2289737

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1280272-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity.PMID 1280272Read ↗

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